LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1964..2060) /label=attL2 /note="attL2 (c)" misc_signal complement(2075..2094) /note="T7 Promoter/priming site (c)" primer_bind 2102..2118 /note="M13 Reverse priming site" gene 2231..3040 /note="Kanamycin resistance gene" rep_origin 3161..3834 /note="pUC origin" PCR_frame 669..1964 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..1984) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1981) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1972..3837,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1942..1962 /note="TEV site" source 673..1941 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:3194 IMAGE:3357207" /tissue_type="Eye, retinoblastoma" /clone_lib="NIH_MGC_16" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..1941 /gene="PIGM" /gene_synonym=MGC29896 /db_xref="GeneID:93183" /db_xref="HGNC:18858" CDS 673..1941 /dnas_title="phosphatidylinositol glycan anchor biosynthesis, class M" /gene="PIGM" /gene_synonym=MGC29896 /codon_start=1 /product="phosphatidylinositol glycan anchor biosynthesis, class M" /protein_id="AAH01803.1" /db_xref="GI:45708486" /db_xref="GeneID:93183" /db_xref="HGNC:18858" /translation="MGSTKHWGEWLLNLKVAPAGVFGVAFLARVALVFYGVFQDRTLH VRYTDIDYQVFTDAARFVTEGRSPYLRATYRYTPLLGWLLTPNIYLSELFGKFLFISC DLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSRGNADSIVASLVLMVLYL IKKRLVACAAVFYGFAVHMKIYPVTYILPITLHLLPDRDNDKSLRQFRYTFQACLYEL LKRLCNRAVLLFVAVAGLTFFALSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFY MLYLTAESKWSFSLGIAAFLPQLILLSAVSFAYYRDLVFCCFLHTSIFVTFNKVCTSQ YFLWYLCLLPLVMPLVRMPWKRAVVLLMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLA GLFFLLINCSILIQIISHYKEEPLTERIKYD" misc_feature 673..1941 /note="PIGM coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgggctc caccaagcac tggggcgaat ggctcctgaa cttgaaggtg 721 gctccagccg gcgtctttgg tgtggccttt ctagccagag tcgccctggt tttctatggc 781 gtcttccagg accggaccct gcacgtgagg tatacggaca tcgactacca ggtcttcacc 841 gacgccgcgc gcttcgtcac ggaggggcgc tcgccttacc tgagagccac gtaccgttac 901 accccgctgc tgggttggct cctcactccc aacatctacc tcagcgagct ctttggaaag 961 tttctcttca tcagctgcga cctcctcacc gctttcctct tataccgcct gctgctgctg 1021 aaggggctgg ggcgccgcca ggcttgtggc tactgtgtct tttggcttct taaccccctg 1081 cctatggcag tatccagccg cggtaatgcg gactctattg tcgcctccct ggtcctgatg 1141 gtcctctact tgataaagaa aagactcgtc gcgtgtgcag ctgtattcta tggtttcgcg 1201 gtgcatatga agatatatcc agtgacttac atccttccca taaccctcca cctgcttcca 1261 gatcgcgaca atgacaaaag cctccgtcaa ttccggtaca ctttccaggc ttgtttgtac 1321 gagctcctga aaaggctgtg taatcgggct gtgctgctgt ttgtagcagt tgctggactc 1381 acgttttttg ccctgagctt tggtttttac tatgagtacg gctgggaatt tttggaacac 1441 acctactttt atcacctgac taggcgggat atccgtcaca acttttctcc gtacttctac 1501 atgctgtatt tgactgcaga gagcaagtgg agtttttccc tgggaattgc tgcattcctg 1561 ccacagctca tcttgctttc agctgtgtct ttcgcctatt acagagacct cgttttttgt 1621 tgttttcttc atacgtccat ttttgtgact tttaacaaag tctgcacctc ccagtacttt 1681 ctttggtacc tctgcttact gcctcttgtg atgccactag tcagaatgcc ttggaaaaga 1741 gctgtagttc tcctaatgtt atggtttata gggcaggcca tgtggctggc tcctgcctat 1801 gttctagagt ttcaaggaaa gaacaccttt ctgtttattt ggttagctgg tttgttcttt 1861 cttcttatca attgttccat cctgattcaa attatttccc attacaaaga agaacccctg 1921 acagagagaa tcaaatatga cgaaaacttg tactttcaag gctCCAGCTT TCTTGTACAA 1981 AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG 2041 TCAAAATAAA ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT 2101 GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC 2161 ACAAGATAAA ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC 2221 AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT 2281 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC 2341 AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG 2401 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT 2461 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC 2521 TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA 2581 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG 2641 TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG 2701 TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG 2761 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT 2821 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG 2881 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT 2941 TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA 3001 TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT 3061 GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT GACCAAAATC 3121 CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 3181 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 3241 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 3301 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA 3361 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 3421 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 3481 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 3541 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC 3601 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 3661 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 3721 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 3781 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT //